Imaging Center Services

Available Services » Tissue Processing : Negative Staining : Liquid Nitrogen

Tissue Processing (Embedding & Sectioning)

Reichert Jung UltraCut E Microtome

lab equipment

Tissue can be embedded is Spurr's resin, Epon, or Epon/Araldite blends, as well as LRWhite for immunocytochemistry. This lab has experience processing many types of tissue, tissue slices, tissue cultures, and pellets. A variety of embedment methods are available, such as Beem or gelatin capsules, flat molds, and flat embedment.

Thin Sectioning

Thin sections, ranging from 50nm to 200nm in thickness, are routinely cut on the Reichert Jung UltraCut E microtome. Many types of grids are available to accommodate the investigators needs.

Semi-Thin Sectioning

Semi-thin sections, also called thick sections, are readily cut on the Reichert Jung UltraCut E microtome, using glass knives. Counter-staining with Toluidine Blue is available.


To watch the preparation of serial sections, click on the image to the left and then click on the play button at the bottom left of the video.

Location, Price, and Scheduling

Located on the 3rd floor of the Link building. Please visit the web calendar to schedule equipment use. New users, please contact Amanda Preston at 448-5976 or by apresto9@uthsc.edu for instructions and signup.

[ Return to Top ]

Negative Staining

Negative staining can be done in the lab using uranyl acetate or phosphotungstic acid and viewed immediately on the electron microscope.

[ Return to Top ]

Liquid Nitrogen Availability

Two large tanks of liquid nitrogen are on hand for those investigators who need it on the occasional basis.

[ Return to Top ]


Pricing Information

For questions contact:

Amanda Preston
Technical Manager
Phone: (901) 448-5976
Email: apresto9@uthsc.edu


Contact Us

Neuroscience Institute
University of Tennessee Health Science Center
875 Monroe Ave, Suite 426
Memphis, TN 38163
Phone: (901) 448-5960
Fax: (901) 448-4685

Physical Address
426 Wittenborg Anatomy Building

Director:
William E. Armstrong, Ph.D.

Co-Director:
Anton J. Reiner, Ph.D.

Administrative Services Assistant:
Shannon Guyot

Website:
Brandy Fleming, M.S.

The Neuroscience Institute - University of Tennessee Health Science Center

The Neuroscience Institute on Facebook