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Services and Fees

UTHSC Investigators: Before preparing the samples, please read the sample preparation guidelines and requirements for an application of interest or contact the facility. Initial consultations are free.

Examples of Consultation Services Offered (Fees may apply):

  • Research project design
  • Sample preparation, processing recommendations, experimental design
  • Letters of support, assistance in grant application
  • Assistance in manuscript preparation

Fee Structure for UTHSC Internal and External Users

 
  • UTHSC Internal Users – Please use fee schedule for services listed below to calculate total service cost
  • External Users - Please use fee schedule for services listed below to calculate total service cost and then add 50%
Sample Preparation Services
Services Service Code Unit $$/unit (FY18-19)
Agilent QC test - up to 10 amples per chip 100 per chip 51.50
In-gel trypsin digestion 101 per band 41.20
In-solution trypsin digestion 102  per sample 41.20
In-solution LysC/trypsin digestion 102a   per sample 61.80
Reduction/alkylation 103   per sample 20.60
Desalting of peptide mixtures - ZipTip 104   per sample 10.30
Detergent removal/clean-up through acetone precipitation of proteins 105   per sample 20.60
Protein concentration - BCA assay in triplicates - at least 3 samples 106 per sample 20.60
Peptide (mixture) consentration - BCA assay in triplicates - at least 3 samples 107 per sample 20.60
High pH Reversed-Phase (HpHRP) Fractionation - 8 step fractions 110 per sample

164.80

Complete sample processing starting from cleared cell lysate, membrane pellets, or cell pellets   
Processing for following LC/MS/MS analysis 111 set-up 206.00
  111a per sample 51.50
Processing for following label-free differential protein expression analysis 112 set-up 206.00
  112a per sample 51.50
Processing for following TMT/iTRAQ labeling 113 set-up 206.00
  113a per sample 51.50
TMT/iTRAQ labeling/preparation for LC and MS analysis, 4-plex (set of 4 samples) 114 per set 721.00
TMT/iTRAQ labeling/preparation for LC and MS analysis, 6-plex (set of 6 samples) 116 per set 1,030.00
TMT/iTRAQ labeling/preparation for LC and MS analysis, 8-plex (set of 8 samples) 118 per set 1,287.50
TMT/iTRAQ labeling/preparation for LC and MS analysis, 10-plex (set of 10 samples) 119 per set 1,390.50
Mass Spectrometry Services - Direct Infusion
Services Service Code Unit $$/Unit (FY18-19)
Determination of the Molecular Mass: More Information here.
Infusion ESI-HRAM MS analysis - small analyte 201 per sample 51.50
Intact protein 202 per sample 82.40
Identification of Small Analyte
Infusion ESI-MS/MS analysis, spcified transition 203 per sample 82.40
Mass Spectrometry Services - Proteomics
Service Service Code Unit

$$/Unit (FY18-19)

Protein Identification: More Information here.

LC/MS/MS, DB search (does not include sample digestin/preparation)

RP LC/MS/MS - 30 min gradient 300 per sample 72.10
RP LC/MS/MS - 60 min gradient 301 per sample 113.30
RP LC/MS/MS - 120 min gradient 302 per sample 185.40
RP LC/MS/MS - 240 min gradient 304 per sample 309.00
MudPIT, DB search (does not include sample digestin/preparation)
6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 310 per fraction 144.20
7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 311 per fraction 139.50
>12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 312 per fraction 133.90

Protein Identification and Mapping of Specified PTMs: More Information here.

LC/MS/MS, DB/PTM search (does not include sample digestin/preparation)

RP LC/MS/MS - 60 min gradient 401 per sample 113.30
RP LC/MS/MS - 120 min gradient 402 per sample 185.40
RP LC/MS/MS - 240 min gradient 403 per sample 309.00
Search for specified PTMs 404 per PTM 30.90

Differential Protein Expression Analysis - iTRAQ/TMT Based Analysis (Reporter Ion quantification): More Information here.

LC/MS/MS, DB search/quantification (does not include sample labeling/preparation)

Unfractionated mixture of labeled peptides - 2 hr RP-LC/MS/MS 500 per run 206.00
Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS 510 per run 333.90

MudPIT, DB search/quantification (does not include sample labeling/preparation)

6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 501 per fraction 154.50
7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 502 per fraction 149.35
>12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 503 per fraction 144.20

Differential Protein Expression Analysis - SILAC Analysis (Precursor Ion Quantification): More Information here.

LC/MS/MS, DB search/quantification (does not include sample labeling/preparation)

Unfractionated mixture of labeled peptides - 2 hr RP-LC/MS/MS 500 per run 206.00
Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS 510 per run 339.90

MudPIT, DB search/quantification (does not include sample labeling/preparation)

6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 511 per fraction 154.50
7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 512 per fraction 149.35
>12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 513 per fraction 144.20

Differential Protein Expression Analysis - Label Free Analysis (Precursor ion Peak Area Detection): More Information here.

RP-LC/HRAM-MS/MS, post-acquisition analysis (does not include sample digestion/preparation)

RP-LC/HRAM-MS/MS - 120 min gradient 521 per run 206.00
RP-LC/HRAM-MS/MS - 240 min gradient 522 per run 339.90

Absolute Quantification of Specified (Target) Proteins - Parallel Reaction Monitoring (PRM)

PRM analysis for peptide quantification (does not include sample digestin/preparation)

RP LC/MS/MS, post-acquisition analysis, Peptide Internal Standard, AUC quantification

RP LC/MS/MS - peptide quantification, 30 min gradient 531 per run 82.40
RP LC/MS/MS - peptide quantification, 60 min gradient 532 per run 128.75
RP LC/MS/MS - peptide quantification, 120 min gradient 533 per run 206.00
Mass Spectrometry Services - Metabolomics

Service

Service Code Unit $$/Unit (FY18-19)

Absolute Quantification of Specified Metabolyte/Small Molecule

LC/MS/MS, post-acquisition analysys, Calibration Curve, Internal Standard, AUC

QuantificationInternal and calibration standards are provided by the customer

Standard Run - LC/MS/MS 15 min 601 per run 17.51
Extended Run - LC/MS/MS 30 min 602 per run 30.90

Differential Quantitative Profiling of Metabolytes - Untargeted Analysis

LC/HRAM-MS/MS, post-acquisition analysis, HRAM AUC Quantification, MS/MS identification

Standard Run - LC/HRAM-MS/MS 15 min 611 per run 30.90
Extended Run - LC/HRAM-MS/MS 30 min 612 per run 51.50

Finding Expected Compound/Transformation - Targeted Analysis

RP-LC/HRAM-MS/MS, post-acquisition analysis, MS/MS identification

Standard Run - LC/HRAM-MS/MS 15 min 621 per run 30.90
Extended Run - LC/HRAM-MS/MS 30 min 622 per run 51.50

Additional Metabolic Resources:

 

Last Published: Mar 12, 2021