AIC Equipment
Super Resolution Microscopy (SRM) Unit
- Zeiss Elyra 7 equipped with dual pco.edge sCMOS cameras, 4 Laser lines (405, 488,
561, 642) and 4 Objective lenses:
- EC-Plan Neofluar 10x/0.30 M27
- Plan-Aprochromat 40x/1.4 Oil DIC M27
- Plan-Aprochromat 63x/1.4 Oil DIC M27
- alpha Plan-Aprochromat 63x/1.46 Oil Corr M27
- The Zeiss Elyra 7 system is capable of 2D and 3D wide-field microscopy, Lattice Structured
Illumination Microscopy (SIM), Total Internal Reflectance Fluorescence Microscopy
(TIRFM), and Single-Molecule Localization Microscopy (SMLM).
- Lattice SIM allows very light-efficient imaging resulting in less photodamage to cells. Key features
include:
- 2D imaging speeds up to 255 fps
- Image up to 4 channels at a time
- Resolution down to 120 nm laterally, 300 nm axially
- Lattice SIM allows very light-efficient imaging resulting in less photodamage to cells. Key features
include:
- SMLM utilizes photo-switchable fluorescent dyes and proteins to induce spontaneous
“blinking” of single fluorophores. This allows investigation of subcellular structures
and single molecules in both fixed and live cells. Key features include:
- Dual camera capability to image two different fluorophores at once
- Resolution down to 20nm laterally and 50 nm axially
- View the sample preparation guide for the Elyra.
- View the AIC's Zeiss Elyra 7 Super Resolution Microscopy (SRM) Flyer
