Ying Kong, Ph.D.
858 Madison Ave.
401 Molecular Science Building
Memphis, TN 38163
Our laboratory is interested in the molecular microbiology and immunology of tuberculosis (TB). TB is an infectious disease mainly caused by Mycobacterium tuberculosis. It remains a leading public health problem worldwide. Our primary goal is to better understand the pathogenesis of this disease. We have developed a variety of non-invasive optical in vivo imaging tools to study TB in animal models. By using non-invasive real-time imaging technologies to monitor TB in small animals, infections can be visualized within individual animals over time, without requiring euthanasia to determine the location and quantity of bacteria. It allows comprehensive examination of all tissues and organs, rather than sampling of sites previously known to be infected, so it may discover important events that we could neglect using samples from discontinued time points. It helps reducing number of experimental animals by obtaining data from numerous time points without having to sacrificing animals to sample tissues in the middle of observation, and thus reducing the cost. Combining these in vivo imaging tools with other cutting-edge in vitro technologies, we will study host-pathogen interactions in M. tuberculosis infected animals and cell lines to identify bacterial and host factors affecting bacterial infection, survival, persistence, and reactivation. The results from these studies will contribute to development of improved diagnostic tools, anti-tuberculosis therapies and vaccines.
- Xie, H*, Mire, J*, Kong, Y*, Chang, M, Hassounah, HA, Thornton, CN, Sacchettini, JC, Cirillo, JD, Rao, J.Rapid point-of-care detection of the tuberculosis pathogen using a BlaC-specific fluorogenic probe. Nat Chem, 4 (10), 802-9, 2012. (Have contributed equally)
- Zhou, Y, Kong, Y, Kundu, S, Cirillo, JD, Liang, H.Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. J Nanobiotechnology, 10, 19, 2012.
- Kong, Y, Akin, AR, Francis, KP, Zhang, N, Troy, TL, Xie, H, Rao, J, Cirillo, SL, Cirillo, JD. Whole-body imaging of infection using fluorescence. Curr Protoc Microbiol, Chapter 2, Unit 2C.3, 2011.
- Kong, Y, Shi, Y, Chang, M, Akin, AR, Francis, KP, Zhang, N, Troy, TL, Yao, H, Rao, J, Cirillo, SL, Cirillo, JD. Whole-body imaging of infection using bioluminescence. Curr Protoc Microbiol, Chapter 2, Unit 2C.4, 2011.
- Mufti, N*, Kong, Y*, Cirillo, JD, Maitland, KC. Fiber optic microendoscopy for preclinical study of bacterial infection dynamics. Biomed Opt Express, 2 (5), 1121-34, 2011. (Have contributed equally)
- Feng, Y*, Kong, Y*, Barnes, PF, Huang, FF, Klucar, P, Wang, X, Samten, B, Sengupta, M, Machona, B, Donis, R, Tvinnereim, AR, Shams, H. Exposure to cigarette smoke inhibits the pulmonary T-cell response to influenza virus and Mycobacterium tuberculosis. Infect Immun, 79 (1), 229-37, 2011. (Have contributed equally)
- Kong, Y, Cirillo, JD. Reporter enzyme fluorescence (REF) imaging and quantification of tuberculosis in live animals. Virulence, 1 (6), 558-62, 2010.
- Kong, Y, Yao, H, Ren, H, Subbian, S, Cirillo, SL, Sacchettini, JC, Rao, J, Cirillo, JD. Imaging tuberculosis with endogenous beta-lactamase reporter enzyme fluorescence in live mice. Proc Natl Acad Sci U S A, 107 (27), 12239-44, 2010.
- Kong, Y, Subbian, S, Cirillo, SL, Cirillo, JD.Application of optical imaging to study of extrapulmonary spread by tuberculosis. Tuberculosis (Edinb), 89 Suppl 1, S15-7, 2009.
- Klucar, P, Barnes, PF, Kong, Y, Howard, ST, Pang, X, Huang, FF, Tvinnereim, AR, Samten, B, Shams, H.Vaccination strategies to enhance local immunity and protection against Mycobacteriun tuberculosis. Vaccine, 27 (12), 1816-24, 2009.
- Klucar, P, Barnes, PF, Kong, Y, Samten, B, Tvinnereim, A, Spallek, R, Nepom, GT, Singh, M, Shams, H. Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen. Hum Immunol, 69 (8), 475-83, 2008.
- Kong, Y, Cave, MD, Zhang, L, Foxman, B, Marrs, CF, Bates, JH, Yang, ZH. Association between Mycobacterium tuberculosis Beijing/W lineage strain infection and extrathoracic tuberculosis: Insights from epidemiologic and clinical characterization of the three principal genetic groups of M. tuberculosis clinical isolates. J Clin Microbiol, 45 (2), 409-14, 2007.
- Kong, Y, Cave, MD, Zhang, L, Foxman, B, Marrs, CF, Bates, JH, Yang, ZH. Population-based study of deletions in five different genomic regions of Mycobacterium tuberculosis and possible clinical relevance of the deletions. J Clin Microbiol, 44 (11), 3940-6, 2006.
- Kong, Y, Cave, MD, Yang, D, Zhang, L, Marrs, CF, Foxman, B, Bates, JH, Wilson, F, Mukasa, LN, Yang, ZH. Distribution of insertion- and deletion-associated genetic polymorphisms among four Mycobacterium tuberculosis phospholipase C genes and associations with extrathoracic tuberculosis: a population-based study. J Clin Microbiol, 43 (12), 6048-53, 2005.
- Yang, Z, Yang, D, Kong, Y, Zhang, L, Marrs, CF, Foxman, B, Bates, JH, Wilson, F, Cave, MD. Clinical relevance of Mycobacterium tuberculosis plcD gene mutations. Am J Respir Crit Care Med, 171 (12), 1436-42, 2005.
- Yang, Z, Kong, Y, Wilson, F, Foxman, B, Fowler, AH, Marrs, CF, Cave, MD, Bates, JH. Identification of risk factors for extrapulmonary tuberculosis. Clin Infect Dis, 38 (2), 199-205, 2004.
- Ph.D. University of Michigan
- Postdoc: University of Texas Health Science Center Texas A & M Health Science Center