Next Generation Sequencing

Next Generation Sequencing Request Form

For pricing and experimental design, contact Bill Taylor. For sample handling, contact Caitlin Costelle.

  314 Chip 316 Chip 318 chip P1 Chip P2 Chip
Coverage 300K-550K reads 1-3 Million reads 2-5 Million reads 35-80 Million reads Coming 2014
Data File Size 30-100 MB 300 Mb-1 GB 500 Mb-2 GB ~10 GB Coming 2014
# samples # samples can be barcoded depends on coverage required
RNA Seq   1 µg of poly(A) RNA or rRNA depleted RNA or 2 µg of DNase treated RNA, low input samples contact us
chIPseq 300 pg-30 ng of chIP DNA, platform used dependent on number of samples being run, low input samples (contact us
miRNAseq 2 µg of enriched miRNA (for enrichment of miRNA contact us)
Platform used will depend on the number of samples submitted.
Targeted Seq 200 ng-1 µg genomic DNA, platform used will depend on the number of samples.
Exomes   2 µg of genomic DNA
Whole Genome Sequencing   500 pg-2 µg genomic DNA, best to wait till P2 chip available for increased coverage (low input contact us)
Special Instructions Prior arrangements must be made for data analysis; RNA samples must be DNase treated before submitting; Samples must be quantified by fluorescence.
All RNA samples must have a RIN of 8 or better from Agilent Bioanalyzer, for low input samples contact us.