Next Generation Sequencing
|314 Chip||316 Chip||318 chip||P1 Chip||P2 Chip|
|Coverage||300K-550K reads||1-3 Million reads||2-5 Million reads||35-80 Million reads||Coming 2014|
|Data File Size||30-100 MB||300 Mb-1 GB||500 Mb-2 GB||~10 GB||Coming 2014|
|# samples||# samples can be barcoded depends on coverage required|
|RNA Seq||1 µg of poly(A) RNA or rRNA depleted RNA or 2 µg of DNase treated RNA, low input samples contact us|
|chIPseq||300 pg-30 ng of chIP DNA, platform used dependent on number of samples being run, low input samples (<2 µg) contact us|
|miRNAseq||2 µg of enriched miRNA (for enrichment of miRNA contact us)|
Platform used will depend on the number of samples submitted.
|Targeted Seq||200 ng-1 µg genomic DNA, platform used will depend on the number of samples.|
|Exomes||2 µg of genomic DNA|
|Whole Genome Sequencing||500 pg-2 µg genomic DNA, best to wait till P2 chip available for increased coverage (low input <2 µg contact us)|
|Special Instructions||Prior arrangements must be made for data analysis; RNA samples must be DNase treated before submitting; Samples must be quantified by fluorescence.|
All RNA samples must have a RIN of 8 or better from Agilent Bioanalyzer, for low input samples contact us.