Infectious Diseases Laboratory
The mission of the Pediatric Infectious Disease Laboratory (PIDL) is to address public health concerns of the citizens of our state by providing infrastructure and administrative support for multidisciplinary and coordinated research, teaching, and community service programs; to foster epidemiological and biomedical research and training in infectious diseases; and to encourage collaborative research efforts addressing infectious disease problems of mutual concern.
Critical Care fellows and faculty members have contributed significantly to the clinical and basic science research performed in the PIDL for over 10 years. In the last 3 years, CCM researchers improved a monocyte cell line sepsis -inflammation model. They examined the gene expression differences between community-associated vs. hospital-acquired Methicillin-Resistant strains of Staphylococcus Aureus (MRSA). Specifically, they analyzed the role of Phenol-Soluble Modulin-alpha (PSM-a) and Delta-toxin of Community-Associated MRSA (CA-MRSA) on macrophage stimulation.
Subsequently, in 2009 and 2010, they conducted a series of experiments to examine suppressors of the inflammatory reaction in the same model. They discovered that Ketamine suppresses the TNF a -stimulated macrophage response after MRSA stimulation and confirmed that lytic antibiotics (like daptomycin) activate a diminished inflammatory response in comparison to non-lytic antibiotics like Vancomycin. This differential response was additive to the suppressive effects of Ketamine and was mediated via NMDA receptors.
CCM Faculty and Fellows have presented these data successfully in the 2009 and 2010 Annual Congresses of the Society of Critical Care Medicine (SCCM), at the 2009 Annual Meeting of the Pediatric Academic Societies, and at the 25th anniversary meeting (2009) of the Section of Critical Care in American Academy of Pediatrics. .
Department of Pediatrics
Critical Care Division
LeBonheur Children's Hospital
50 N Dunlap, 3rd Floor
Memphis, TN 38103