Imaging Center

The Neuroscience Imaging Center is located on the third floor of the Link building. It provides services in light and electron microscopy to faculty, staff and graduate students at the University of Tennessee, as well as the Memphis research community and private institutions nationwide.

Operating on a cost recovery basis, it offers use of equipment and technical services in the areas of

Available »

Transmission Electron Microscopy

Desmosome

20,000X image of mouse heart. Desmosome, the junction between cardiomyocytes, shown by red arrow. Courtesy of Dr. Enkhe Purevjav and Dr. T.J. Hollingsworth.

RPE Phagocytosis

50,000X image of rat retinal pigment epithelium and rod photoreceptor. Phagocytosis of rod outer segment shown by red arrow. Courtesy of Dr. Tony Reiner and Dr. T.J. Hollingsworth.

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Immunogold localization of neurophysins in dense core granules of vasopressin and oxytocin nerve terminals. Courtesy Dr. William E. Armstrong

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Immunogold staining for GABA axodendritic synapses. Courtesy Dr. William E. Armstrong

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Confocal Microscopy

Cerebellar Purkinje

Mouse cerebellar Purkinje cells labeled for calbindin (red) and proline-rich transmembrane protein 2 (PRRT2, green). Nuclei labeled with DAPI (blue). 20X objective. Courtesy of Dr. Moshahid Khan and Dr. T.J. Hollingsworth.

Hippocampus

Mouse hippocampus labeled for microtubule-associated protein 2 (MAP2, red), PRRT2 (green), and neuronal nuclei (NeuN, blue). Total nuclei stained with DAPI (white). 20X objective. Courtesy of Dr. Moshahid Khan and Dr. T.J. Hollingsworth.

Retina

Mouse retina labeled for vesicular glutamate transporter 1 (vGlut1, red), PRRT2, and glutamate receptor 1A (GluR1). Nuclei stained with DAPI (white). 40X oil objective. Courtesy of Dr. Moshahid Khan and Dr. T.J. Hollingsworth.

Breast epithelial cells

Human breast epithelial cells labeled for beta-actin (red) and beta-tubulin (green). Nuclei stained with DAPI (white). 63X oil objective. Courtesy of Pallabita Chowdhury and Dr. T.J. Hollingsworth.

Mouse retina

Mouse retina labeled for ADP-ribosylation factor like GTPase 13b (Arl13B, red), acetylated tubulin (green), and protein kinase C (PKC, blue). Nuclei stained with DAPI (white). 40X oil objective. Courtesy of Dr. Nawajes Mandal and Dr. T.J. Hollingsworth.

Mouse retina

Mouse retina labeled for Arl13b (red), acetylated tubulin (green), and PKC (blue). Nuclei stained with DAPI (white). 63X oil objective. Courtesy of Dr. Nawajes Mandal and Dr. T.J. Hollingsworth.

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Rat hypothalamo-neurohypophysial system. Localization of oxytocin (red) and vasopressin neurons and axons. Courtesy Dr. William E. Armstrong

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Colocalization of GFAP (red) and SK3 channels (green). Overlap is yellow. Courtesy Dr. William E. Armstrong

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Confocal, immunofluorescence image of potassium channel subunits Kv2.1 (red) and Kv 2.2 (green). Courtesy Dr. William E. Armstrong

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Confocal microscopic image of GABA-containing terminals (red) in close apposition to oxytocin neurons (green) in rat hypothalamus. Courtesy Dr. William E. Armstrong

 

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Courtesy Dr. Larry Reiter

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Sagittal brain section of a transgenic adult mouse brain showing GAD65,67-EGFP and Thy-1 (green) neurons, and dopamine neurons stained for tyrosine hydroxlyase (red). Courtesy Dr. Fu-Ming Zhou

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Somatosensory cortical area pyramidal neurons and their apical dendrites labeled with yellow fluorescent protein. Image was taken with a 40X oil objective. Courtesy Dr. Fu-Ming Zhou

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A segment of an apical dendrite of a cortical pyramidal neuron labeled with yellow fluorescent protein. Note the numerous dendritic spines. Image was taken with a 40X oil objective. Courtesy Dr. Fu-Ming Zhou


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Dense hippocampal CA1 area pyramidal neurons and their downward-pointing apical dendrites labeled with yellow fluorescent protein. Image was taken with a 40X oil objective. Courtesy Dr. Fu-Ming Zhou


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Mouse cerebellar Purkinje cells labeled by GAD1-controlled GFP. Image was taken with a 20X objective. Courtesy Dr. Fu-Ming Zhou

 Pyramidal 

Pyramidal neurons in the cerebral cortex. Courtesy Dr. Fu-Ming Zhou

 Hippocampal

Mouse hippocampal neurons labeled with GFP. Imaged with a 20X objective on Zeiss 710. Courtesy Dr. Fu-Ming Zhou

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Drosophila 3rd instar larval neuromuscular junction stained with DLG (Disks Large) to identify synaptic boutons and Nc-82 (Bruchpilot) to identify syaptic active zones. Courtesy Dr. Larry Reiter

 

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Neuron Mapping & 3-D Tracing

Mouse retina

3D surface reconstruction of Arl13b (red), acetylated tubulin (green), and PKC (blue) labeled mouse retina on 63X oil objective performed using IMARIS analysis software. Courtesy of Dr. Nawajes Mandal and Dr. T.J. Hollingsworth.

Mouse retina

3D surface reconstruction and cytoskeletal filament trace of Arl13b (red, surface), acetylated tubulin (green, filament trace), and PKC (blue, surface) labeled mouse retina on 63X oil objective performed using IMARIS analysis software. Courtesy of Dr. Nawajes Mandal and Dr. T.J. Hollingsworth.

Mouse retina

3D surface reconstruction and cytoskeletal filament trace of Arl13b (red, surface) and acetylated tubulin (green, filament trace) labeled mouse retina on 63X oil objective performed using IMARIS analysis software. Zoomed to show acetylated tubulin network in photoreceptor cilia. Courtesy of Dr. Nawajes Mandal and Dr. T.J. Hollingsworth.

Pyramidal spines

3D filament trace of a pyramidal neurons dendrites (red) with spines labeled (blue) performed using IMARIS analysis software. Courtesy of Dr. T.J. Hollingsworth.

Breast epithelial cell

3D filament trace of breast epithelial cell beta-tubulin cytoskeleton (green) with surface rendering of beta-actin (red) and nuclei (blue) performed using IMARIS analysis software. Courtesy of Pallabita Chowdhury and Dr. T.J. Hollingsworth.

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Courtesy Microbright Field, Inc.

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Courtesy Microbright Field, Inc.

 

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Pricing Information

For questions contact:

Esther Marquez Wilkins, PhD
Technical Manager
Phone: (901) 448-5976
Email: emarque2@uthsc.edu

Contact Us

Neuroscience Institute
University of Tennessee Health Science Center
875 Monroe Ave, Suite 426
Memphis, TN 38163
Phone: (901) 448-5960
Fax: (901) 448-4685

Physical Address
311 Suite Link Building

Director:
William E. Armstrong, PhD

Co-Director:
Anton J. Reiner, PhD

Administrative Aide:
Mistie Brewer

Program Coordinator/
Website:

Brandy Fleming, MS

Technical Manager:
Esther Marquez Wilkins, PhD

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