The PixCell II ™ Laser Capture Microdissection system includes: a solid state near infrared laser diode with <7.5 µ m, 15 µ m, and 30 µ m spot sizes; the Controller power supply (0-100 mW maximum); an Olympus IX70 inverted microscope with 4x, 10x, 20x, and 40x objectives; an Hitachi KPD-580 color CCD video camera; a Matrox Meteor™ color frame grabber board; and a Sony Trinitron video monitor. The Fluor-300 fluorescence system is comprised of: a 100W mercury arc lamp; bluefilter cube (Ex 455-495 nm/Em >510 nm); green filter cube (Ex 503-547 nm/Em >565 nm); red filter cube (Ex 590-650 nm/Em >667 nm); and an IR blocking filter for the camera and a Sony Trinitron monitor.
The Arcturus ® XT system include: a Nikon Eclipse® Ti-E microscope base; infrared (IR) Capture Laser: Solid-state, near-IR (810 nm); UV Cutting Laser: Solid-state, passive Q-switched, diode-pumped (355 nm) ; 2x, 10x, and 40x Nikon CFI60 objectives ; motorized stage with 1 µ m precision, and the up-to-date software.
More system details available at the LifeTech website.
Laser Capture Microdissection Facility
Hao Chen, Ph,D.
Department of Pharmacology
Nash Annex, Room 250F
Memphis, TN 38163