Instrument

The PixCell II ™ Laser Capture Microdissection system includes: a solid state near infrared laser diode with <7.5 µ m, 15 µ m, and 30 µ m spot sizes; the Controller power supply (0-100 mW maximum); an Olympus IX70 inverted microscope with 4x, 10x, 20x, and 40x objectives; an Hitachi KPD-580 color CCD video camera; a Matrox Meteor™ color frame grabber board; and a Sony Trinitron video monitor. The Fluor-300 fluorescence system is comprised of: a 100W mercury arc lamp; bluefilter cube (Ex 455-495 nm/Em >510 nm); green filter cube (Ex 503-547 nm/Em >565 nm); red filter cube (Ex 590-650 nm/Em >667 nm); and an IR blocking filter for the camera and a Sony Trinitron monitor.

The Arcturus ® XT system include: a Nikon Eclipse® Ti-E microscope base; infrared (IR) Capture Laser: Solid-state, near-IR (810 nm); UV Cutting Laser: Solid-state, passive Q-switched, diode-pumped (355 nm) ; 2x, 10x, and 40x Nikon CFI60 objectives ; motorized stage with 1 µ m precision, and the up-to-date software.

More system details available at the LifeTech website.  external link icon

Laser Capture Microdissection Facility

Hao Chen, Ph,D.
Director
Assistant Professor
Department of Pharmacology
Nash Annex, Room 250F
Memphis, TN 38163
Phone: 901-448-3720
Email: hchen@uthsc.edu