UTHSC Viral Vector Core Laboratory

UTHSC Viral Vector Core Laboratory (VVC) was established with support from a Department of Defense grant awarded in May 2007. Located in the Cancer Research Building on the Memphis campus of UTHSC, the VVC provides services to the Memphis, Knoxville and Chattanooga campuses. Viral Vector Core services include lentiviral, adenoviral and adeno-associated viral vector, as well as traditional retroviral vectors. Vectors services are available to all UTHSC investigators to support their research efforts on a fee-for-service basis.

Viral Vector Request Form

The most advanced Lentiviral vectors with capacity to deliver 7 kbp of your gene sequence.
Lentiviral vectors are derived by deletion of the replication genes from human HIV, a member of the Retroviridae virus family. The deletions make the vectors safe and provide space for gene inserts.

Lentiviral vectors from the Vector Core offer:

  • Extraordinarily efficient gene delivery - 100% of cells are infected in most cases.
  • Infection of all mammalian species and cell types using the Vesicular Stomatitis Virus glycoprotein (VSV-G) to coat the vectors.
  • Gene delivery to non-dividing and dividing cells.
  • Regulated, tissue-specific and constitutive overexpression of your gene.
  • Immediate stable expression because your genes are inserted into host cell chromosomes within hours after infection.
  • Create gene knock-downs using shRNA or miRNA in animals and in cell culture - these can also be regulated or tissue-specific!
  • Generate transgenic mice and rats using Lentiviral vectors.
  • Third generation self-inactivating (SIN) vectors for increased safety.

The most advanced Adenoviral vectors with capacity to deliver up to 7 kbp of your gene sequence.
Adenoviral vectors are derived from human adenovirus serotype 5 by deletion of the early gene E1 and E3 regions. The gene of interest is usually inserted in the E1 region of adeno-genome.

Adenoviral vectors from the Vector Core offer:

  • Extraordinarily efficient gene delivery - 100% of cells are infected in most cases.
  • Infection of many types of human and rodent cells and many cell types in vivo.
  • Gene delivery to non-dividing and dividing cells.
  • Very high levels of gene expression that can last for three to six months.
  • Regulated and constitutive overexpression of your gene.
  • Create gene knock-downs in cell culture using miRNA - these can also be regulated!