Services Provided
Following are examples of flow cytometric analyses available:
- Differentiation of relative cell size and complexity for eukaryotic and prokaryotic cells
- Simultaneous detection and quantification of expression of up to 11 cell surface and/or intracellular molecules with flurophore-labeled antibodies
- Ca2+ flux measurement among cell subpopulations defined by multiple cell-surface markers
- Detection and quantification of GFP-labeled protein expression
- Ca2+ flux during cell signaling among cell subpopulations defined by cell surface markers
- DNA quantification and cell cycle analysis
- Detection and quantification of apoptosis
- High Throughput Screening (HTS) of cells or flurophore-labeled beads for the detection of cytokines or cell signaling phosphoproteins, i.e. BD Cytometric Bead Array (CBA)
Following are examples of cell sorting services available:
- High-speed sorting of cells identified with up to 12 cell surface and/or intracellular fluorescent markers into four or fewer defined subpopulations
- Detection and high-speed sorting of bacteria or eukaryotic subcellular particles
- Sorting into microwell plates
- Single-cell deposition into microwell plates or onto microscope slides
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Lawrence M. Pfeffer, Ph.D.
Interim Vice Chancellor
Office of Research
910 Madison, Suite 608
Memphis, TN 38163
Phone: (901) 448-7125
Fax: (901) 448-7133
research@uthsc.edu
