Tissue Collection for DNA Analysis
Tail-snipping is a common procedure to obtain tissue to use as a source of genomic
DNA for genetic analysis and monitoring in mice. Mice are altricial when born and
undergo significant developmental changes in the first 4 weeks of life, including
an increase in the vascularity, innervation and mineralization of bone in the tail.
UTHSC IACUC policy allows the collection of <5 mm of tail tip without anesthesia in
mice <3 weeks of age. Tail snipping of mice older than 3 weeks of age must be performed
with anesthesia and analgesics.
Our veterinary technicians are trained in performing tail snips and use sterile instruments and will apply an anticoagulant to the tail following the procedure. Mice can also be ear tagged at the same time so that the DNA results can be individually identified. Each PI that wants tail snipping to be performed must have the procedure described in an approved IACUC protocol. The PI is expected to provide Eppendorf tubes (other another suitable container) for storage of tail snips.
Timothy D. Mandrell, DVM, DACLAM
Director, Laboratory Animal Care Unit
956 Court, Room B106
Memphis, TN 38163