Nanodrop, Qubit, and SpectraMax M2e Plate Reader
|Nanodrop||Qubit||SpectraMax M2e Plate Reader|
Concentration of Nucleic Acid
Concentration of RNA or DNA
|Absorbance, Florescence, Luminescence
RNA, DNA, Proteins
(Accuracy diminishes less than 20 ng/µl and above 1500 ng/µl)
|RNA Broad Range: 20-1000 ng
RNA: 5-100 ng
DNA Broad Range: 2-1000 ng
DNA HS: 0.2-100 ng
|Depends on the assay type|
|Advantages||A260/A280 ratios and A260/A230 ratios;
Able to read 1 sample or 8 samples at a time
|Usually not affected by organics, High accuracy and sensitivity||Able to read both 96-well plates and cuvette. Flexible platform; able to read nucleic acids and proteins. Very sensitive. Reads top and bottom, and has thermal regulation from 4°C to 45°C.|
|Disadvantages||Concentrations affected by contaminants||Can’t tell purity of sample||Purity of samples can affect readings|
|Special Instructions||Samples should be in water or low TE.
Nucleic acid samples only.
Contaminated samples will need to be purified.
|Needs ~15 min preparation time||First time users should be trained on software. MRC does not provide cuvette or plates. For kinetic assay, please write down the start and finish time.|