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MRC Announcements

2/23/2010

The Molecular Resource Center is pleased to announce the following additions to the current services available to provide high throughput processing and analysis.

Two SOLiD next generation sequencers have been added. These next generation sequencers support a wide range of applications including:

Genome:
De Novo Sequencing, Targeted
Resequencing, Whole Genome Resequencing

Transcriptome:
 Gene Expression Profiling, Small RNA
Analysis, Whole Transcriptome Analysis

Epigenome:
Chromatin Immunoprecipitation
Sequencing (ChIP Seq)

Methylation Analysis
Due to the complexity of these processes, the MRC requires a consultation with MRC personnel before submitting projects for the next generation sequencers.

The Fluidigm Biomark Array System will be available Spring 2010.

The Fluidigm Biomark Array System uses microfluidic chips to achieve high sample throughput by parallel amplification of 96 unique samples for expression analysis, genotyping, and digital PCR. Up to 96 samples are mixed with 96 primer pair sets, resulting in 9216 reactions. The samples are analyzed as conventional qPCR reactions, effectively becoming a PCR microarray. These samples are also sequencer ready, allowing base-pair level analysis. A user's existing PCR protocols, reagents, and primers are used as-is on the chips. Every reaction combines both an amplicon tagging and a barcoding (identification) step that enables all amplicons to be multiplexed for sequencing. Combining this technology with the MRC's existing robotic sample handling for capillary and SOLiD sequencing would allows users to rapidly analyze large RNA and DNA sample sets in hours rather than weeks and months. In addition to time saving, the considerably reduced volume of reagents and primers consumed by the microfluidics technology allows the user to realize significant cost savings per sample.

Affymetrix Microarray services upgraded
 With the addition of a new Affy chip scanner, the MRC is now capable of providing a full service Affy microarray facility. For information on Affy microarrays please contact Lorne Rose.

The new Eppendorf Liquid Handling Robot allows investigators to prepare large numbers of samples for processing through RTPCR, SOLiD and DNA sequencing.

The Qiagen Qiacube allows for fully automated purification of nucleic acids and proteins for subsequent PCR, microarray and sequencing.

11/10/2009

Jump Starting Genomics in Your Research Program: A How To

By

Rob Williams, Ph.D.
Director

Center for Integrative and Translational Genomics

UTHSC has a strong program in mouse genetics and genomics that you and your research team can almost certainly exploit in ongoing research and your next grant application. This is true whatever species or disease process you study. The Center for Integrative and Translational Genomics has preliminary results that you are likely to be able to use. In particular we have a great deal of gene expression data for mouse, rat, and human. If your research involves any level of molecular biology (proteins, mRNAs, small metabolites), you will almost certainly be able to get something useful out of the CITG GeneNetwork system. In this talk Rob Williams, Director of the CITG and of GeneNetwork will walk you through the process of extracting, analyzing, and incorporating data sets that are available on-line to all UTHSC researchers.

BRING your research problems or favorite genes and proteins to this work seminar.

11/10/2009 (10:30am-11:30pm)

GEB Room A304

Get Your Free Mice Here! A Practical Guide to Genomics and Personalized Medicine

Sponsored by the UT Center for Integrative and Translational Genomics Genomics and the UT Molecular Resource Center

11/4/2009

Jump Starting Genomics in Your Research Program: A How To

By

Rob Williams, Ph.D.
Director

Center for Integrative and Translational Genomics

UTHSC has a strong program in mouse genetics and genomics that you and your research team can almost certainly exploit in ongoing research and your next grant application. This is true whatever species or disease process you study. The Center for Integrative and Translational Genomics has preliminary results that you are likely to be able to use. In particular we have a great deal of gene expression data for mouse, rat, and human. If your research involves any level of molecular biology (proteins, mRNAs, small metabolites), you will almost certainly be able to get something useful out of the CITG GeneNetwork system. In this talk Rob Williams, Director of the CITG and of GeneNetwork will walk you through the process of extracting, analyzing, and incorporating data sets that are available on-line to all UTHSC researchers.

BRING your research problems or favorite genes and proteins to this work seminar.

11/04/2009 (9:30am - 10:30am)

Coleman Building

South Auditorium

Get Your Free Mice Here! A Practical Guide to Genomics and Personalized Medicine

Sponsored by the UT Center for Integrative and Translational Genomics and the UT Molecular Resource Center

October 2009

Pipette Repair Service

October 26 – October 28

(Monday through Wednesday)

If you would like to have your pipette(s) serviced bring them to

Drop-off Location:

The UTHSC General Stores (basement of the GEB) between 8:00 a.m. and 5:00 p.m.

Please drop them off as early as possible

Drop off deadline:
no later than noon
Wednesday, 10/28

September 2009

Applied Biosystems applications specialist Charles Cochran will be presenting a seminar on the SOLiD 3 System and Applications on Friday, September 18 from 11:00 am to 12:00 pm in room A102 of the GEB in conjunction with the CTSI Research Technology Unit Core Facilities Open House.

Did you know UTHSC provides access to some of the most advanced scientific technologies available today?

If you would like to discover how you can access these technologies for your research projects, then make plans to attend the CTSI Research Technology Core Open House on Friday, September 18 from 12-2 pm in the lobby of the General Education Building at the corner of Madison and Dunlap.

Clinical Translational Science Institute

Research Technology Cores

Open House

When: September 18, 2009 from 12:00 – 2:00 pm
Where: GEB lobby (corner of Madison and Dunlap)

Research technology Core participants:

Biomedical Engineering Mass Spectrometry Core
Biomedical Instrumentation Micro CAT/SPECT Imaging Core
Biomedical Informatics Unit Microbial Pathogenesis/IVIS/RBL
Cancer Center IVIS Imaging Molecular Resource Center
CTSI Front Porch Molecular Science Confocal Facility
Drug Discovery and Development Neuroscience Inst. Imaging Facility
Flow Cytometry and Cell Sorting Core Viral Vector Core

Representative from the following Scientific Technology companies will be available to discuss applications/use of their technologies.

Applied Biosystems SOLiD
Illumina Microarrays
Qiagen
Transnetyx Automated Mouse Genotyping

May 27, 2009

Open House
Time: 2:00 - 4:00 p.m.

The Molecular Resource Center recently upgraded some equipment and has added some new services for the UTHSC campus. Come by and see these new technologies and talk with our staff about what we can do to enhance your research projects and make your projects easier.

Roche and Affymetrix reps will be available to answer your questions concerning their products and services.

January 2009

MRC/TransnetYX, Inc. Automated Genotyping

Contact: Ryan Yanase, TransnetYX, Inc.

UT Molecular Resource Center (MRC) has negotiated an agreement with TransnetYX to provide fully automated genotyping services to University of Tennessee researchers at a discounted price.

TransnetYX has developed a proprietary technology and high throughput process for DNA extraction and analysis. Their automated genotyping service screens for transgenic, knock-in, knock-out, and single base changes in tissue using real time PCR. Results are reported electronically within 72 hours.

For more information and to sign up for a 25 sample free trial visit:
http://mailyourtail.com/utmrc

November 2007

MRC announces collaboration with the Vision Research Core of the Hamilton Eye Institute (Illumina Microarray)