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Services and Fees

UTHSC Investigators: Before preparing the samples, please read the sample preparation guidelines and requirements for an application of interest or contact the facility. Initial consultations are free.

Examples of Consultation Services Offered (Fees may apply):

  • Research project design
  • Sample preparation, processing recommendations, experimental design
  • Letters of support, assistance in grant application
  • Assistance in manuscript preparation

Fee Structure for UTHSC Internal and External Users

 
  • UTHSC Internal Users – Please use fee schedule for services listed below to calculate total service cost
  • External Users - Please use fee schedule for services listed below to calculate total service cost and then add 50%
Sample Preparation Services
Services Service Code Unit $$/unit
Agilent QC test - up to 10 amples per chip 100 per chip 50
In-gel trypsin digestion 101 per band 40
In-solution trypsin digestion 102  per sample 40
In-solution LysC/trypsin digestion 102a   per sample 60
Reduction/alkylation 103   per sample 20
Desalting of peptide mixtures - ZipTip 104   per sample 10
Detergent removal/clean-up through acetone precipitation of proteins 105   per sample 20
Protein concentration - BCA assay in triplicates - at least 3 samples 106 per sample 20
Peptide (mixture) consentration - BCA assay in triplicates - at least 3 samples 107 per sample 20
High pH Reversed-Phase (HpHRP) Fractionation - 8 step fractions 110 per sample

160

Complete sample processing starting from cleared cell lysate, membrane pellets, or cell pellets   
Processing for following LC/MS/MS analysis 111 set-up 200
  111a per sample 50
Processing for following label-free differential protein expression analysis 112 set-up 200
  112a per sample 50
Processing for following TMT/iTRAQ labeling 113 set-up 200
  113a per sample 50
TMT/iTRAQ labeling/preparation for LC and MS analysis, 4-plex (set of 4 samples) 114 per set 700
TMT/iTRAQ labeling/preparation for LC and MS analysis, 6-plex (set of 6 samples) 116 per set 1000
TMT/iTRAQ labeling/preparation for LC and MS analysis, 8-plex (set of 8 samples) 118 per set 1250
TMT/iTRAQ labeling/preparation for LC and MS analysis, 10-plex (set of 10 samples) 119 per set 1350
Mass Spectrometry Services - Direct Infusion
Services Service Code Unit $$/Unit
Determination of the Molecular Mass: More Information here.
Infusion ESI-HRAM MS analysis - small analyte 201 per sample 50
Intact protein 202 per sample 80
Identification of Small Analyte
Infusion ESI-MS/MS analysis, spcified transition 203 per sample 80
Mass Spectrometry Services - Proteomics
Service Service Code Unit $$/Unit

Protein Identification: More Information here.

LC/MS/MS, DB search (does not include sample digestin/preparation)

RP LC/MS/MS - 30 min gradient 300 per sample 70
RP LC/MS/MS - 60 min gradient 301 per sample 110
RP LC/MS/MS - 120 min gradient 302 per sample 180
RP LC/MS/MS - 240 min gradient 304 per sample 300
MudPIT, DB search (does not include sample digestin/preparation)
6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 310 per fraction 140
7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 311 per fraction 135
>12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 312 per fraction 130

Protein Identification and Mapping of Specified PTMs: More Information here.

LC/MS/MS, DB/PTM search (does not include sample digestin/preparation)

RP LC/MS/MS - 60 min gradient 401 per sample 110
RP LC/MS/MS - 120 min gradient 402 per sample 180
RP LC/MS/MS - 240 min gradient 403 per sample 300
Search for specified PTMs 404 per PTM 30

Differential Protein Expression Analysis - iTRAQ/TMT Based Analysis (Reporter Ion quantification): More Information here.

LC/MS/MS, DB search/quantification (does not include sample labeling/preparation)

Unfractionated mixture of labeled peptides - 2 hr RP-LC/MS/MS 500 per run 200
Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS 510 per run 330

MudPIT, DB search/quantification (does not include sample labeling/preparation)

6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 501 per fraction 150
7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 502 per fraction 145
>12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 503 per fraction 140

Differential Protein Expression Analysis - SILAC Analysis (Precursor Ion Quantification): More Information here.

LC/MS/MS, DB search/quantification (does not include sample labeling/preparation)

Unfractionated mixture of labeled peptides - 2 hr RP-LC/MS/MS 500 per run 200
Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS 510 per run 330

MudPIT, DB search/quantification (does not include sample labeling/preparation)

6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 511 per fraction 150
7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 512 per fraction 145
>12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction 513 per fraction 140

Differential Protein Expression Analysis - Label Free Analysis (Precursor ion Peak Area Detection): More Information here.

RP-LC/HRAM-MS/MS, post-acquisition analysis (does not include sample digestion/preparation)

RP-LC/HRAM-MS/MS - 120 min gradient 521 per run 200
RP-LC/HRAM-MS/MS - 240 min gradient 522 per run 330

Absolute Quantification of Specified (Target) Proteins - Parallel Reaction Monitoring (PRM)

PRM analysis for peptide quantification (does not include sample digestin/preparation)

RP LC/MS/MS, post-acquisition analysis, Peptide Internal Standard, AUC quantification

RP LC/MS/MS - peptide quantification, 30 min gradient 531 per run 80
RP LC/MS/MS - peptide quantification, 60 min gradient 532 per run 125
RP LC/MS/MS - peptide quantification, 120 min gradient 533 per run 200
Mass Spectrometry Services - Metabolomics

Metabolomics:

Additional Metabolic Resources:

 

Last Published: Oct 31, 2017