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Illumina NextSeq500

Illumina NextSeq500

Next Seq 500 v2 kits: NextSeq 500 High Output v2 (75 cycles; "single end" reads NextSeq 500 High Output v2 (150 cycles; 75bp x 2 "paired end" reads) NextSeq 500 High Output v2 (150 cycles; "single end" reads NextSeq 500 High Output v2 (300 cycles; 150 bp x 2 "paired end" reads only)
Coverage Up to 400 million reads. Best suited for sequencing DNA from one end (chIP-seq), microbiome or miRNA sequencing  Up to 400 million reads. Paired-end sequencing facilitates detection of genomic rearrangments and repetitive sequence elements, as well as gene fusions and novel transcripts. Also required for de novo sequencing.  Up to 400 million reads. Suitable for whole genome sequencing (WGS), whole transcriptome sequencing, mRNA-seq, exome sequencing Up to 400 million reads. Paired-end sequencing facilitates detection of genomic rearrangments and repetitive sequence elements, as well as gene fusions and novel transcripts
Data File Size 25-30 GB 50-60 GB 50-60 GB 100-120 GB
Number of Samples The number of smaples depends on coverage required. Please consult the MRC and the mBio core to design experiment
RNA-seq   2 µg of DNase-treated RNA; low input samples contact us


ChIP-seq 1.5 ng-30 ng of ChIP DNA, low input samples (<100 ng) contact us
miRNA-seq 2 µg of enriched miRNA or 3 µg of total RNA (contact us)
Targeted Sequencing 200 ng-1 µg genomic input; may be possible to use the Mid Throughput v2 kits (130 million reads) contact us
Exomes    2 µg of genomic DNA
Whole genome Sequencing   500 ng-2 µg genomic DNA
Special Instructions

Please consult the mBio core for experimental design and data analysis consultation; ***RNA samples must be DNase-treated before submitting;***
Samples must be quantified by fluorescence.
All RNA samples must have a minimum RIN of 8 by Agilent Bioanalyzer, assay; for low input samples contact us.
Customers must sign to agree to the quote attached to the sample submission form before the work begins. 

Last Published: Sep 30, 2016