Jonathan H. Jaggar, Ph.D. Laboratory

Projects

Research in the lab studies regulation, physiological functions, and pathological alterations in arterial smooth muscle cell plasma membrane and intracellular ion channels and local and global intracellular Ca2+ signals. We use a multi-faceted approach that incorporates many molecular, cellular, and whole artery techniques. Molecular biology and biochemical techniques include cloning, siRNA/shRNA, adenoviral expression, RT-PCR, Western blotting, recombinant protein expression, biotinylation, FRET imaging, immunofluorescence and co-immunoprecipitation. We use patch-clamp and conventional electrophysiology, high speed confocal Ca2+ imaging, ratiometric Ca2+ imaging, and pressurized arterial diameter myography. Genetic knockout and cardiovascular disease models are used.

Some of our recent projects include:

  • Physiological functions and pathological alterations of arterial smooth muscle cell IP3 receptors mediated through calcium signal regulation and physical coupling to TRP channels.
  • Arterial smooth muscle cell voltage-dependent Ca2+ channel auxiliary subunits in health and disease.
  • Mitochondrial modulation of arterial smooth muscle cell calcium signaling, gene expression, ion channel activity, and contractility.
  • Vasoregulation by the physiological gas transmitters carbon monoxide and hydrogen sulfide.

Contact Us

Office

Jonathan H. Jaggar, Ph.D.
Department of Physiology
University of Tennessee Health Science Center
Memphis TN 38163
Phone: 901-448-1208
Fax: 901-448-7126

Shipping Address

Room 426, Nash Building
Department of Physiology
University of Tennessee Health Science Center
894 Union Ave
Memphis TN 38163