Shared Analytical Instrument Specifications

Varian Inova 500 MHz

Manufacturer Agilent (Santa Clara California, USA)
Specifications
  • 3-channel Inova 500 console (1 high-band (1H/19F) channel, 2 broadband)
  • 500 MHz Oxford magnet
  • Varian L-700 Pulsed Gradient Driver
  • Anti-vibration legs
  • Variable temperature module
  • PC with Varian LC- NMR software
  • Gilson Liquid handler for LC-NMR
Probes
  • 5mm TRPFG (triple resonance pulsed field gradient)-H, C, N, Inverse detection (ID, optimized for 1H)
  • 5mm S/W PFG (switchable)
  • 5mm ID Penta PFG (1H, 13C, 15N, 31P, and 2H)
  • 3mm ID IFC (interchangeable Flow Cell) trpfg probe, can change from flow to tube, good for small amount samples (
  • 4mm Nano probe (HRMAS-High Resolution Magic Angle Spinning, for paramagnetic samples and semi-solid samples such as cells/tissues )
Software Linux based VNMRJ
Applications
  • Routine 1D, 2D NMR
  • Variable temperature experiments (-20 to +80oC)
  • Identification and characterization small amount (
  • Advanced applications using 4mm nano probe for semi-solids and paramagnetic nano particle samples

Bruker Avance III 400 MHz

Manufacturer BrukerBiospin (Billerica, MA, USA)
Specifications
  • Nanobay console
  • 400 MHz magnet
  • Bruker SampleCase (autosampler) with 24 sample holders
  • Anti-vibration legs
  • Ultra-shielded and ultra-long hold magnet
  • Variable temperature experiments
  • Automatic Tuning and Matching (ATM)
  • Fully automated operation
Probes
  • 5mm BBO (Broad Band Observe) probe
  • The inner coil is optimized for x-nuclei from15N to 31P and with z-gradient
Software Topspin 3.0 on Windows 7
Applications
  • Routine 1D, 2D NMR
  • Variable temperature experiments (-20 to +80C)
  • Multiple sample acquisition

Waters Xevo G2-S Q-TOF

Manufacturer Waters (Milford, MA, USA)
Ionization modes Positive and negative (ESI and APCI by direct infusion or LCMS, and Solid Analysis Probe)
Analyzer/detector
  • Sensitivity enhancement by Step-wave technology
  • Mass Selective Quadrupole MS
  • Travelling Wave Ion Guide (TWIG) collision cell
  • Orthogonal TOF (Time of Flight)
Operating modes
  • Full scan MS with two point lock mass correction
  • MSe (fragment all ions)
  • MSMS (fragment a selected parent ion)
M/Z range 20-100,000 Da
Resolving power 30,000 FWHM
Mass Accuracy ±1ppm
Inlets Waters Acquity I-Class UPLC including:
  • Sample Manager (holds 2 x 48 vials or 2 x 96-well plates)
  • Binary Solvent Manager (flow range: 0.1 to 2.0/min (gradient))
  • Thermostatted Column Manager
  • PDA (Photo Diode Array) detector
Software Masslynx 4.1 on Windows 7
Applications
  • High-resolution analysis
  • Identification of unknown metabolites
  • Forensics
  • Quantitative analysis of both small and big molecules

AB Sciex API-3000

Manufacturer AB Sciex (Foster City, CA, USA)
Ionization modes Positive and negative (ESI and APCI) by direct infusion or LCMS
Analyzer Triple quadrupole
Operating modes
  • MS Full-scan (Q1 or Q3)
  • MS/MS Product ion Scan
  • MS/MS Precursor ion Scan
  • MS/MS Neutral Loss Scan
  • Multiple-Reaction Monitoring (MRM)
M/Z range Up to 2000 Da
Resolving power Unit mass resolution
Mass Accuracy ±0.2u
Inlets
  • Shimatzu 10Avp HPLC (flow range: from 0.1 to 2.0 mL/min)
  • CTC PAL Autosampler (holds 6 x 48 vials or 96-well plates)
Software Analyst 1.5.1 on Windows 7
Applications
  • Low-resolution analysis for small molecules
  • MS/MS for both qualitative and quantitative analysis of small molecules

AB Sciex API-4500

Manufacturer AB Sciex (Foster City, CA, USA)
Ionization modes Positive and negative (Turbo V source with ESI and APCI) by direct infusion or LCMS
Analyzer Triple quadrupole (QJet® 2 Ion Guide delivers 10x higher sensitivity over API-3000)
Operating modes
  • MS Full-scan (Q1 or Q3)
  • MS/MS Product ion Scan
  • MS/MS Precursor ion Scan
  • MS/MS Neutral Loss Scan
  • Multiple-Reaction Monitoring (MRM)
M/Z range Up to 2000 Da
Resolving power Unit mass resolution
Mass Accuracy ±0.2u
Inlets
  • Shimadzu Nexera X2 UPLC (flow range: from 0.1 to 2.0 mL/min)
  • CTC PAL Autosampler (holds 6 x 48 vials or 96-well plates)

Bruker Esquire LC

Manufacturer Bruker Daltonics (Billerica, MA, USA))
Ionization modes Positive and negative (ESI and APCI) by direct infusion or LCMS
Analyzer Ion trap
Operating modes
  • Full scan MS
  • MSMS
M/Z range Up to 6000 Da
Resolving power Unit mass resolution
Mass Accuracy ±0.2u
Inlets Agilent 1100 HPLC including
  • G1322A Degasser
  • G1312A Binary HPLC Pump with SSV (flow range: from 0.1 to 1 mL/min)
  • G1313A ALS Autosampler (holds 100 samples)
  • G1314A VWD Variable Wavelength Detector (range: 190nm to 390nm)
  • G1315A DAD Detector
  • G1321A FLD(Fluorescence detector)
  • G1316A Heated Column Compartment
  • Waters 2424 ELS detectors
Software ChemStation and Esquire on Windows XP
Applications
  • Low-resolution analysis (nominal mass) for small molecules and peptides
  • Qualitative and quantitative analysis of small molecules

Contact Us

Wei Li, Ph.D.
Professor and Faculty Director of Instrument Facility
University of Tennessee Health Science Center
881 Madison Avenue, room 561
Memphis, TN 38163
901-448-7532 (office)
Email: wli@uthsc.edu

Dejian Ma, PhD
Senior Research Specialist
University of Tennessee Health Science Center
881 Madison Avenue, room 560
Memphis TN 38163
Email: 
dma6@uthsc.edu 
Phone: 901-448-1550

Contact Us

Wei Li, PhD
Professor and Faculty Director of Instrument Facility
University of Tennessee Health Science Center
881 Madison Avenue, room 561
Memphis, TN 38163
(901) 448-7532 (office)
Email: wli@uthsc.edu

Dejian Ma, PhD
Senior Research Specialist
University of Tennessee Health Science Center
881 Madison Avenue, room 560
Memphis TN 38163
Email: dma6@uthsc.edu 
Phone: (901) 448-1550