Jian Zuo, Ph.D.
Department of Developmental Neurobiology
St. Jude Children's Research Hospital
Department of Anatomy and Neurobiology
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105
Tel: (901) 495-3891
Fax: (901) 495-2270
Email: Jian Zuo
- Ph.D. Institution: University of California, San Francisco, CA (1993)
- Postdoctoral: Rockefeller University
The work in Dr. Zuo's laboratory is focused on the identification and characterization of genes important for the development and degeneration of cerebellar Purkinje cells and inner ear hair cells. They are taking two complimentary approaches. The first, positional cloning of mouse mutant genes, takes advantage of recent advances in the mouse genome project. Mouse cerebellar mutants, such as lurcher (Lc), Purkinje cell degeneration (pcd) and nervous (nr), are of particular interest. They have identified and characterized the Lc gene and established genetic and physical maps of pcd and nr loci. As the mutant pcd gene induces Bcl-2 dependent apoptosis, they are searching for downstream genes involved in the degenerative process in pcd mutant mice. The second approach takes advantage of a new technique that allows modification of endogenous genes in bacterial artificial chromosomes (BACs) with a green fluorescent protein (GFP) reporter gene and further introduction of modified BACS into pronuclei to generate trans-genic mice with labeled neurons in the developing brain. They have chosen to modify three genes that have been shown to be expressed specifically in hair cells of the inner ear: alpha-9 AchR, Brn3.1 and delta-1 GluR. These transgenic mice will provide a resource for the construction of hair cell specific cDNA libraries at different developmental stages, as well as a tool for the introduction of exogenous genes into hair cells in vivo and for making hair cell specific knockout mice.
- Liu Z, Fang J, Dearman J, Zhang L, Zuo J. In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic atoh1 expression. PLoS One. 2014 Feb 20;9(2):e89377. doi: 10.1371/journal.pone.0089377. eCollection 2014. PubMed PMID: 24586731; PubMed Central PMCID: PMC3930725.
- Cox BC, Chai R, Lenoir A, Liu Z, Zhang L, Nguyen DH, Chalasani K, Steigelman KA, Fang J, Cheng AG, Zuo J. Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development. 2014 Feb;141(4):816-29. doi: 10.1242/dev.103036. PubMed PMID: 24496619; PubMed Central PMCID: PMC3912828.
- Layman WS, Sauceda MA, Zuo J. Epigenetic alterations by NuRD and PRC2 in the neonatal mouse cochlea. Hear Res. 2013 Oct;304:167-78. doi: 10.1016/j.heares.2013.07.017. Epub 2013 Aug 2. PubMed PMID: 23911933; PubMed Central PMCID: PMC3784354.
- Yang JJ, Lim JY, Huang J, Bass J, Wu J, Wang C, Fang J, Stewart E, Harstead EH, E S, Robinson GW, Evans WE, Pappo A, Zuo J, Relling MV, Onar-Thomas A, Gajjar A, Stewart CF. The role of inherited TPMT and COMT genetic variation in cisplatin-induced ototoxicity in children with cancer. Clin Pharmacol Ther. 2013 Aug;94(2):252-9. doi: 10.1038/clpt.2013.121. Epub 2013 Jun 11. PubMed PMID: 23820299; PubMed Central PMCID: PMC3883563.
- Liu Z, Liu Z, Walters BJ, Owen T, Kopan R, Zuo J. In vivo visualization of Notch1 proteolysis reveals the heterogeneity of Notch1 signaling activity in the mouse cochlea. PLoS One. 2013 May 31;8(5):e64903. doi: 10.1371/journal.pone.0064903. Print 2013. PubMed PMID: 23741415; PubMed Central PMCID: PMC3669271.
- Mellado Lagarde MM, Cox BC, Fang J, Taylor R, Forge A, Zuo J. Selective ablation of pillar and deiters' cells severely affects cochlear postnatal development and hearing in mice. J Neurosci. 2013 Jan 23;33(4):1564-76. doi: 10.1523/JNEUROSCI.3088-12.2013. PubMed PMID: 23345230; PubMed Central PMCID: PMC3567488.