Dominic M. Desiderio, Ph.D.
Department of Neurology
The University of Tennessee Health Science Center
847 Monroe Avenue
Memphis, TN 38163
Tel: (901) 448-5488
Fax: (901) 448-7842
Lab: 117 Johnson Building
Email: Dominic M. Desiderio
- Ph.D. Institution: Massachusetts Institute of Technology, Department of Chemistry
The hypothesis for this study is that differentially expressed nitroproteins (DENPÕs) exist between human pituitary controls and adenomas. We base our hypothesis on our recent discovery of sixteen 3-nitrotyrosine-containing proteins (3NT proteins; nitroproteins) and several nitroprotein:protein complexes in human pituitaries. Biomarker DENPÕs provide a window into oxidative stress, reactive nitrogen species (RNS), reactive oxygen species (ROS), free radicals, and tumorigenesis. We focus on brain tumors, and specifically on pituitary adenomas. The molecular mechanisms that participate in the formation of non-secreting macroadenomas are largely unknown; more is known about hormone-secreting microadenomas.
Although reversible and non-reversible protein modifications occur, we focus on a chemically stable end-product biomarker, 3-nitro tyrosine (3NT). We analyze nitroproteins in human pituitary tissues (post-mortem controls; post-surgical adenomas) to identify DENPÕs. Those biomarker DENPÕs will clarify molecular events that occur in brain tumors and pituitary adenomas. Those results are readily translatable to other cancer studies.
We have extensive preliminary data on human pituitaries from collaborations with our neurosurgeon colleagues J. T. Robertson, M.D. (University of Tennessee) and Nelson M. Oyesiku, M.D. (Emory University). We have published the quantitation of neuropeptides (controls; adenomas), comparative proteomics to discover differentially expressed proteins (DEPÕs), qualitative phosphoproteomics to discover phosphoproteins, and qualitative nitroproteomics to discover nitroproteins. We have compared our DEPÕs with differential genes.
- Kim DK, Kang B, Kim OY, Choi DS, Lee J, Kim SR, Go G, Yoon YJ, Kim JH, Jang SC, Park KS, Choi EJ, Kim KP, Desiderio DM, Kim YK, LÃ¶tvall J, Hwang D, Gho YS. EVpedia: an integrated database of high-throughput data for systemic analyses of extracellular vesicles. J Extracell Vesicles. 2013 Mar 19;2. doi: 10.3402/jev.v2i0.20384. PubMed PMID: 24009897; PubMed Central PMCID: PMC3760654.
- Zhan X, Wang X, Desiderio DM. Pituitary adenoma nitroproteomics: current status and perspectives. Oxid Med Cell Longev. 2013;2013:580710. doi: 10.1155/2013/580710. Epub 2013 Mar 7. Review. PubMed PMID: 23533694; PubMed Central PMCID: PMC3606787.
- Gardner LA, Desiderio DM, Groover CJ, Hartzes A, Yates CR, Zucker-Levin AR, Bloom L, Levin MC. LC-MS/MS identification of the one-carbon cycle metabolites in human plasma. Electrophoresis. 2013 Jun;34(11):1710-6. doi: 10.1002/elps.201200536. Epub 2013 Apr 23. PubMed PMID: 23417555.
- Wu J, Kakoola DN, Lenchik NI, Desiderio DM, Marshall DR, Gerling IC. Molecular phenotyping of immune cells from young NOD mice reveals abnormal metabolic pathways in the early induction phase of autoimmune diabetes. PLoS One. 2012;7(10):e46941. doi: 10.1371/journal.pone.0046941. Epub 2012 Oct 11. PubMed PMID: 23071669; PubMed Central PMCID: PMC3469658.
- Giorgianni F, Mileo V, Desiderio DM, Catinella S, Beranova-Giorgianni S. Characterization of the phosphoproteome in human bronchoalveolar lavage fluid. Int J Proteomics. 2012;2012:460261. Epub 2012 Sep 11. PubMed PMID: 22997577; PubMed Central PMCID: PMC3446654.
- Zhan X, Desiderio DM. Nitroproteins Identified in Human Ex-smoker Bronchoalveolar Lavage Fluid. Aging Dis. 2011 Apr;2(2):100-15. Epub 2010 Nov 8. PubMed PMID: 22396869; PubMed Central PMCID: PMC3295046.