Dominic M. Desiderio, Ph.D.
Department of Neurology
The University of Tennessee Health Science Center
847 Monroe Avenue
Memphis, TN 38163
Tel: (901) 448-5488
Fax: (901) 448-7842
Lab: 117 Johnson Building
Email: Dominic M. Desiderio
- Ph.D. Institution: Massachusetts Institute of Technology, Department of Chemistry
The hypothesis for this study is that differentially expressed nitroproteins (DENPÖs) exist between human pituitary controls and adenomas. We base our hypothesis on our recent discovery of sixteen 3-nitrotyrosine-containing proteins (3NT proteins; nitroproteins) and several nitroprotein:protein complexes in human pituitaries. Biomarker DENPÖs provide a window into oxidative stress, reactive nitrogen species (RNS), reactive oxygen species (ROS), free radicals, and tumorigenesis. We focus on brain tumors, and specifically on pituitary adenomas. The molecular mechanisms that participate in the formation of non-secreting macroadenomas are largely unknown; more is known about hormone-secreting microadenomas.
Although reversible and non-reversible protein modifications occur, we focus on a chemically stable end-product biomarker, 3-nitro tyrosine (3NT). We analyze nitroproteins in human pituitary tissues (post-mortem controls; post-surgical adenomas) to identify DENPÖs. Those biomarker DENPÖs will clarify molecular events that occur in brain tumors and pituitary adenomas. Those results are readily translatable to other cancer studies.
We have extensive preliminary data on human pituitaries from collaborations with our neurosurgeon colleagues J. T. Robertson, M.D. (University of Tennessee) and Nelson M. Oyesiku, M.D. (Emory University). We have published the quantitation of neuropeptides (controls; adenomas), comparative proteomics to discover differentially expressed proteins (DEPÖs), qualitative phosphoproteomics to discover phosphoproteins, and qualitative nitroproteomics to discover nitroproteins. We have compared our DEPÖs with differential genes.
- Zhan X, Desiderio DM, Wang X, Zhan X, Guo T, Li M, Peng F, Chen X, Yang H, Zhang P, Li X, Chen Z. Identification of the proteomic variations of invasive relative to non-invasive non-functional pituitary adenomas. Electrophoresis. 2014 Aug;35(15):2184-94. doi: 10.1002/elps.201300590. PubMed PMID: 24729304.
- Dwivedi N, Neeli I, Schall N, Wan H, Desiderio DM, Csernok E, Thompson PR, Dali H, Briand JP, Muller S, Radic M. Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity. FASEB J. 2014 Jul;28(7):2840-51. doi: 10.1096/fj.13-247254. Epub 2014 Mar 26. PubMed PMID: 24671707.
- Zhan X, Wang X, Desiderio DM. Mass spectrometry analysis of nitrotyrosine-containing proteins. Mass Spectrom Rev. 2013 Dec 6. doi: 10.1002/mas.21413. [Epub ahead of print] PubMed PMID: 24318073.
- Kim DK, Kang B, Kim OY, Choi DS, Lee J, Kim SR, Go G, Yoon YJ, Kim JH, Jang SC, Park KS, Choi EJ, Kim KP, Desiderio DM, Kim YK, LĂ¶tvall J, Hwang D, Gho YS. EVpedia: an integrated database of high-throughput data for systemic analyses of extracellular vesicles. J Extracell Vesicles. 2013 Mar 19;2. doi: 10.3402/jev.v2i0.20384. eCollection 2013. PubMed PMID: 24009897; PubMed Central PMCID: PMC3760654.
- Zhan X, Wang X, Desiderio DM. Pituitary adenoma nitroproteomics: current status and perspectives. Oxid Med Cell Longev. 2013;2013:580710. doi: 10.1155/2013/580710. Epub 2013 Mar 7. Review. PubMed PMID: 23533694; PubMed Central PMCID: PMC3606787.
- Gardner LA, Desiderio DM, Groover CJ, Hartzes A, Yates CR, Zucker-Levin AR, Bloom L, Levin MC. LC-MS/MS identification of the one-carbon cycle metabolites in human plasma. Electrophoresis. 2013 Jun;34(11):1710-6. doi: 10.1002/elps.201200536. Epub 2013 Apr 23. PubMed PMID: 23417555.