Collagen Core

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Collagen Core

David Brand, Director
Phone: 901-577-7282

The Center of Excellence for Diseases of Connective Tissues maintains a core facility for the large-scale purification of collagen, and the production of alpha chains and CB peptides for research in autoimmunity. Tissues used as a source of cartilage are obtained exclusively from meat packing industry by-products.

Presently, these collagens are available from our core service at prices competitive with commercial entities providing this grade of product.

If you are interested in further information regarding pricing and availability
of collagen from our core service, please contact David Brand.

Collagen Amino Acid Sequences

(Click a photo to view a larger image.)

Many grams of cartilage ChipsHomoginization on a large scaleInspecting Carboxymethyl Cellulose Separation of alpha chainsShell-Freezing the desalted collagen productsSublimation on the LyophilyzerFinished Product: 5 ml vials of CII @4 mg/ml in 50mM Acetic AcidCartoon of the CII StructureInflammed Paw of Arthritic MouseWhy we call it Bucket BiochemistryChick Type II Collagen CB PeptidesEmbryonic fibroblast migrating through the developing stromal matrix of a chicken cornea around the 12th day of development ((c) ca. 1970 - D. Hasty)EM of collagen fibrils from chrome tanned cowhide prepared by fragmentation and stained with phosphotungstic acid. The typical axial period is seen as in the shadowed preparation. PTA brings out the characteristic intraperiod structure. Magnification 260,000X. F.O. Schmitt and J. Gross, J. Am. Leather Chem. Assn., XLIII:658,1948