Green flourescent protein + pups

RDRCC Services:

• We perform backcrosses and set up mouse breeding programs.
• We screen using PCR or Flowcytometry for genotyping and to establish homozygosity.
• We offer the following mouse strains:
  • DBA/1 Lac J Tg neg littermates (this stock is limited)
  • DBA/1 Lac J Vb8.3
  • DBA/1 Lac J a/b TCR Tg #24
  • DBA/1 Lac J a/b TCR Tg #27
  • B10.M-DR1 (DRB1*0101)
  • B10.M-DR4 (DRB1*0401)
  • DBA/1 IFN-g KO
  • DBA/1LacJ a/bTCR Tg#24/IFN-gKO
  • DBA/GFP- a/b TCR Tg #24

Order Form 

We have two flow cytometers available:

• FacsCalibur - a four color analyzer with a Multiwell Autosampler (MAS) for high throughput screening
• FacSort - a three color analyzer

We will perform or help you perform the multi-color immunofluorescent staining of cells for analysis.

Fluorochromes Available 

Histogram Examples

Flowcytometry Screening Order Form 

Multiplex Protein Analysis with a Bio-Plex/Luminex instrument

• Quantitates up to 100 proteins simultaneously from a single 100ul samples
• Analyzes Th1/Th2 cytokines, inflammatory mediators, chemokines,
  and others
• Murine or human samples
• Serum or tissue culture samples
• Kits available from a number of vendors

We will perform or help you perform the multi-color immunofluorescent staining of cells for analysis.

Vendors

BD Biosciences
BioRad
Biosource
Linco Research, Inc.
Luminex

Bio-Plex Order Form

Fluorochromes Available 

Histogram Examples

Flowcytometry Screening Order Form 

To Schedule Use of the BioPlex

Call Laura or Nora at (901) 523-8990 ext.7826
E-mail Laura at lmccorm1@uthsc.edu or Nora at nmoore@uthsc.edu.
Stop by the RDRCC/Animal Resource Core and sign up on our calendar posted outside the door.