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Animal Resource Core

The mission at the UTHSC Animal Resource Core is to facilitate the study of rheumatic diseases by providing both expertise in mouse genetics and models of autoimmune arthritis to members of the Rheumatic Diseases Research Core Center (RDRCC) and other interested investigators.

Lab Photos

Bio-plex instrument
Bio-plex instrument

Nora analyzes flowcytometry data
Nora analyzes flowcytometry data

Healthy mice
Healthy mice

Mouse checkup
Mouse checkup


Services and Expertise

Green flourescent protein + pups
Green flourescent protein + pups

RDRCC Services:

  • We perform backcrosses and set up mouse breeding programs.
  • We screen using PCR or Flowcytometry for genotyping and to establish homozygosity.
  • We offer the following mouse strains:
    • DBA/1 Lac J Tg neg littermates (this stock is limited)
    • DBA/1 Lac J Vb8.3
    • DBA/1 Lac J a/b TCR Tg #24
    • DBA/1 Lac J a/b TCR Tg #27
    • B10.M-DR1 (DRB1*0101)
    • B10.M-DR4 (DRB1*0401)
    • DBA/1 IFN-g KO
    • DBA/1LacJ a/bTCR Tg#24/IFN-gKO
    • DBA/GFP- a/b TCR Tg #24

Order FormOpens in Acrobat

We have two flow cytometers available:

  • FacsCalibur - a four color analyzer with a Multiwell Autosampler (MAS) for high throughput screening
  • FacSort - a three color analyzer

We will perform or help you perform the multi-color immunofluorescent staining of cells for analysis.

Fluorochromes AvailableOpens in Acrobat

Histogram Examples

Flowcytometry Screening Order FormOpens in Acrobat

Multiplex Protein Analysis with a Bio-Plex/Luminex instrument

  • Quantitates up to 100 proteins simultaneously from a single 100ul samples
  • Analyzes Th1/Th2 cytokines, inflammatory mediators, chemokines,
    and others
  • Murine or human samples
  • Serum or tissue culture samples
  • Kits available from a number of vendors

We will perform or help you perform the multi-color immunofluorescent staining of cells for analysis.

Vendors

BD Biosciences
BioRad
Biosource
Linco Research, Inc.
Luminex

ELIZA Photo

Bio-Plex Order Form

Fluorochromes AvailableOpens in Acrobat

Histogram Examples

Flowcytometry Screening Order FormOpens in Acrobat


To Schedule Use of the BioPlex

Call Laura or Nora at (901) 523-8990 ext.7826
E-mail Laura at lmccorm1@uthsc.edu or Nora at nmoore@uthsc.edu.
Stop by the RDRCC/Animal Resource Core and sign up on our calendar posted outside the door.

Animal Resource Core - Nora

Contact Us

RDRCC Animal Core

Edward F. Rosloniec, Ph.D.
Director